9/2/2023 0 Comments Genea actimeterNinety percent of the residents were able to take 4 weeks or more for maternity leave. Thirty-one percent had a lower American Board of Surgery In-Training Exam (ABSITE) score. Over half of the residents (55%) were pregnant during their 2nd or 3rd year of residency, with only 18% pregnant during a research year. Seven of the programs (27%) stated that they have had zero residents pregnant. ![]() Each program was asked for the total number of possible responses from female residents that met our criteria (60 female residents). Results An unvalidated survey was sent to 32 general surgery programs and 26 programs responded (81%). The responses were collected and analyzed using the Survey Monkey website. Most questions were in a 5-point Likert scale format. Each program was re-contacted 6 weeks after the initial contact. Each program distributed our survey to the female surgical residents who had been pregnant during residency in the last 5 years. Methods We distributed an electronic, online 26-question survey to 32 general surgery programs in the southwestern region of the United States. The pregnant female surgical resident has an added stress factor compared to her male counterpart. Many medical students decide against surgery as a profession due to the long work hours and family strain. Shifflette, Vanessa Hambright, Susannah Amos, Joseph Darryl Dunn, Ernest Allo, Mariaīackground Surgery continues to be an intense, time-consuming residency. In addition, studies are warranted to explain the role of those proteins in maintaining the pregnancy and Further studies are necessary to define complete plasma protein profile of pregnant female dogs and to identify all proteins that are differentially presented in the pregnant animals compared with the non- pregnant ones. Although we identified only a limited number of differentially presented proteins, our study demonstrated that the plasma protein profile changed during pregnancy in female dogs, which suggests its importance in maintaining pregnancy. Our findings showed that numerous protein spots were differentially presented in the plasma of female dogs during normal pregnancy. In this study, we identified proteins that have not been previously identified in dogs. To the best of our knowledge, this is the first report on the plasma protein profile of pregnant and non- pregnant female dogs. ![]() Five identified protein spots, including zinc finger BED domain-containing protein 5, hemoglobin subunit beta-2, integrator complex subunit 7, apolipoprotein A-I, and glutamyl aminopeptidase were differentially presented in the plasma of pregnant and non- pregnant female dogs. We used matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI TOF MS) to identify 47 spots corresponding to 52 different proteins. The intensity of staining of 35 spots differed significantly (P < 0.05) between the non- pregnant and pregnant female dogs. Analysis of 2-DE gel image exhibited of 249 protein spots. We used the plasma samples obtained from four female dogs during I, II, and III thirds of pregnancy, three days after parturition, as well as from four non- pregnant female dogs in diestrus phase. ![]() ![]() In this study, we used a combined approach based on 2-dimensional electrophoresis (2-DE), difference in gel electrophoresis (DIGE), and mass spectrometry (MS) to identify the plasma protein composition in pregnant female dogs and compared it with non- pregnant female dogs. SzczubiaÅ‚, Marek Wawrzykowski, Jacek DÄ…browski, Roman Krawczyk, Magdalena Kankofer, Marta Preliminary study on plasma proteins in pregnant and non- pregnant female dogs.
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